short inhibiting rna (sirna) dnmt3a-specific sirna (origene)  (OriGene)

Journal: Journal of Gynecologic Oncology

Article Title: ATF1 regulates MAL2 expression through inhibition of miR-630 to mediate the EMT process that promotes cervical cancer cell development and metastasis

doi: 10.3802/jgo.2025.36.e11

Figure Lengend Snippet: ATF1, activating transcription factor 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; si-ATF1, small interfering-activating transcription factor 1; siRNAs, small interfering RNAs; DNMT3A, DNA methyltransferases 3A; si-NC, small interfering negative control; qRT-PCR, quantitative reverse transcription polymerase chain reaction. Relative to the control group: * p<0.01, † p<0.001.

Article Snippet: The siRNAs targeting DNMT3A (si-ATF1) or overexpression vector of MAL2 (oe-MAL2), miR-630 mimic/inhibitor as well as their negative control (NC) group (si-NC, oe-NC, mimic NC, inhibitor NC) were purchased from GenePharma (Shanghai, China).

Techniques: Negative Control, Quantitative RT-PCR, Reverse Transcription, Polymerase Chain Reaction, Control

Journal: Journal of Gynecologic Oncology

Article Title: ATF1 regulates MAL2 expression through inhibition of miR-630 to mediate the EMT process that promotes cervical cancer cell development and metastasis

doi: 10.3802/jgo.2025.36.e11

Figure Lengend Snippet: ATF1, activating transcription factor 1; ChIP, chromatin immunoprecipitation; IgG, immunoglobulin G; si-ATF1, small interfering-activating transcription factor 1; siRNAs, small interfering RNAs; DNMT3A, DNA methyltransferases 3A; si-NC, small interfering negative control; qRT-PCR, quantitative reverse transcription polymerase chain reaction. Relative to the control group: * p<0.01, † p<0.001.

Article Snippet: The siRNAs targeting DNMT3A (si-ATF1) or overexpression vector of MAL2 (oe-MAL2), miR-630 mimic/inhibitor as well as their negative control (NC) group (si-NC, oe-NC, mimic NC, inhibitor NC) were purchased from GenePharma (Shanghai, China).

Techniques: Chromatin Immunoprecipitation, Negative Control, Quantitative RT-PCR, Reverse Transcription, Polymerase Chain Reaction, Control

Journal: Journal of Gynecologic Oncology

Article Title: ATF1 regulates MAL2 expression through inhibition of miR-630 to mediate the EMT process that promotes cervical cancer cell development and metastasis

doi: 10.3802/jgo.2025.36.e11

Figure Lengend Snippet: ATF1, activating transcription factor 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MAL2, myelin and lymphocyte protein 2; NC, negative control; oe-MAL2, overexpression vector of myelin and lymphocyte protein 2; oe-NC, overexpression vector of negative control; si-ATF1, small interfering-activating transcription factor 1; siRNAs, small interfering; DNMT3A, DNA methyltransferases 3A; si-NC, small interfering negative control; qRT-PCR, quantitative reverse transcription polymerase chain reaction. Relative to the control group: * p<0.05, † p<0.01, ‡ p<0.001.

Article Snippet: The siRNAs targeting DNMT3A (si-ATF1) or overexpression vector of MAL2 (oe-MAL2), miR-630 mimic/inhibitor as well as their negative control (NC) group (si-NC, oe-NC, mimic NC, inhibitor NC) were purchased from GenePharma (Shanghai, China).

Techniques: Negative Control, Over Expression, Plasmid Preparation, Quantitative RT-PCR, Reverse Transcription, Polymerase Chain Reaction, Control

Journal: Burns & Trauma

Article Title: p53 promotes the expansion of regulatory T cells via DNMT3a- and TET2- mediated Foxp3 expression in sepsis

doi: 10.1093/burnst/tkad021

Figure Lengend Snippet: Foxp3 expression is regulated by DNMT3a and TET2. ( a , b ) C57BL/6 mice were subjected to sham (n = 5) or CLP (n = 10) procedure and sacrificed after 24 h. Total mRNAs were extracted from splenetic CD4 + T cells and subjected to qPCR analysis of DNMT1, DNMT3a, DNMT3b, TET1, TET2 and TET. β-Actin was used as the loading control. The mRNA levels were normalized to the sham group ( * p < 0.01). ( c ) Total proteins were extracted from cells and used for western blotting of DNMT3a, TET2 and β-actin. The relative expressions were quantified using ImageJ and normalized to that of the loading control ( * p < 0.01, # p < 0.01). ( d ) Jurkat T cells were transfected with lentivirus containing DNMT3a mRNA or siRNA expressing plasmids or blank vector. ( e ) Jurkat T cells were transfected with lentivirus containing TET2 mRNA or siRNA expressing plasmids or blank vector. After stable expression, cells were stimulated with PBS (control) or LPS (1 μg/ml) for 24 h. Then total proteins were extracted from cells and used for western blotting analysis of Foxp3 and β-actin. The relative expression of Foxp3 was quantified using ImageJ and normalized to that of β-actin ( * p < 0.05, * * p < 0.01, # p < 0.01 vs control group in siRNA vector, ## p < 0.01 vs LPS group in siRNA vector). CLP cecal ligation and puncture, m RNA messenger ribonucleic acid, siRNA small interfering ribonucleic acid, qPCR quantitative real-time polymerase chain reaction, DNMT DNA-buffered saline, LPS lipopolysaccharide, Foxp3 forkhead/winged helix transcription factor p3

Article Snippet: Lentivirus vectors expressing the DNA fragments encoding GFP-tagged full-length DNA methyltransferase enzyme (DNMT) 3a, DNMT3a mRNA-targeted small interfering ribonucleic acid (siRNA), ten–eleven translocation 2 (TET2) and TET2 mRNA-targeted siRNA were designed, constructed, packed and purified by Obio Technology Co. Ltd (Shanghai, China).

Techniques: Expressing, Control, Western Blot, Transfection, Plasmid Preparation, Ligation, Real-time Polymerase Chain Reaction, Saline

Journal: Burns & Trauma

Article Title: p53 promotes the expansion of regulatory T cells via DNMT3a- and TET2- mediated Foxp3 expression in sepsis

doi: 10.1093/burnst/tkad021

Figure Lengend Snippet: p53 directly regulates DNMT3a and TET2 expression. ( a , b ) WT and p53 f/f /CD4-Cre + mice were subjected to sham (n = 5) or CLP (n = 10) operation. After 24 h mice were sacrificed and CD4 + T cells were isolated from spleens. ( a ) Cells were subjected to mRNA extraction followed by qPCR analysis of DNMT3a and TET2 mRNAs. β-Actin was used as the loading control. The relative mRNA levels were normalized to that in the sham group ( * p < 0.01). ( b ) Cells were subjected to protein extraction and western blot analysis of DNMT3a and TET2. β-Actin was used as the loading control. The relative expression levels were quantified using ImageJ and normalized to that of the loading control ( * p < 0.01). ( c , d ) Predicted binding sites of p53 on DNMT3a or TET2 promoter regions are indicated with blue arrows or vertical lines. pcDNA3.1- p53 and pGL4.1 carrying WT promoter or mutant promoter with binding sites deletion (left panels) or segment of promoter sequence (right panels) were co-transfected into HEK293T cells. After 3 days, cells were lysed and subjected to luciferase activity detection ( * p < 0.01 vs blank group). CLP cecal ligation and puncture, m RNA messenger ribonucleic acid, qPCR quantitative real-time polymerase chain reaction, DNMT DNA methyltransferase enzyme, TET , ten–eleven translocation, HEK293T human embryonic kidney 293 T cells, WT wild type

Article Snippet: Lentivirus vectors expressing the DNA fragments encoding GFP-tagged full-length DNA methyltransferase enzyme (DNMT) 3a, DNMT3a mRNA-targeted small interfering ribonucleic acid (siRNA), ten–eleven translocation 2 (TET2) and TET2 mRNA-targeted siRNA were designed, constructed, packed and purified by Obio Technology Co. Ltd (Shanghai, China).

Techniques: Expressing, Isolation, Extraction, Control, Protein Extraction, Western Blot, Binding Assay, Mutagenesis, Sequencing, Transfection, Luciferase, Activity Assay, Ligation, Real-time Polymerase Chain Reaction, Translocation Assay